A Mouse Model for Corneal Neovascularization by Alkali Burn

Corneal neovascularization (CoNV), a pathological form of angiogenesis, involves the growth blood and lymph vessels into avascular cornea from limbus adversely affects transparency vision. Alkali burn is one most common forms ocular trauma that leads to CoNV. In this protocol, CoNV experimentally induced using sodium hydroxide solution in controlled manner ensure reproducibility. The alkali model useful for understanding pathology can be extended study angiogenesis general because avascularity, transparency, accessibility cornea. work, was analyzed by direct examination under dissecting microscope immunostaining flat-mount corneas anti-CD31 mAb. Lymphangiogenesis detected on anti-LYVE-1 edema visualized quantified optical coherence tomography (OCT). summary, will help advance existing assays discover new treatment strategies pathologic extraocular angiogenesis.